Journal: Bioengineering

Article Title: SRRM4 Knockout Helps the Human Mesenchymal Stem Cell Line to Penetrate Decellularized Cancellous Bone

doi: 10.3390/bioengineering12121299

Figure Lengend Snippet: DCB intrusion of UE7T-9SRRM4 KO cells. ( A ) UE7T-9 cells extend into decellularized bone. Dotted line is surface of DCB. Upper and lower left: Control shows no extension into the bone trabeculae or bone marrow. Upper and lower right: UE7T-9 SRRM4 KO cells extend continuously along the bone trabeculae. Black arrows: Penetrating UE7T-9 SRRM4 KO cells, Dotted line: DCB Surface, Black bars = 500 µm: upper pictures, 100 µm: lower pictures. ( B ) Distance measurements of penetration in UE7T-9 cells. Left: Distance of cells that extended from the surface of each DCB was measured (NDP viewer). 100 cells were randomly selected. Dotted line is surface of DCB. Black bar = 250 µm Right: In UE7T-9 SRRM4 KO cells, the cells significantly extended into the bone marrow. The measurement of the penetration distance was performed three times using different DCBs from individual porcin ribs. The data from one representative measurement is shown in the figure. ( C ) CXCL12 immunostaining of SRRM4KO UE7T-9 cells extended into the bone matrix (left: HE stained. Right: CXCL12 immunohistochemistry) UE7T-9 SRRM4 KO cells penetrated into the bone ramus show CXCL12 positivity. Black arrows: CXCL12-positive UE7T-9 SRRM4 KO cells, Black bars: 100 µm.

Article Snippet: A primary rabbit monoclonal antibody against anti-CXCL12 (clone D8G6H; Cell Signaling Technology, Danvers, MA, USA) was used in this study.

Techniques: Control, Immunostaining, Staining, Immunohistochemistry

Journal: Bioengineering

Article Title: SRRM4 Knockout Helps the Human Mesenchymal Stem Cell Line to Penetrate Decellularized Cancellous Bone

doi: 10.3390/bioengineering12121299

Figure Lengend Snippet: RNA sequence analysis of UE7T-9 and UE7T-9 SRRM4 UE7T-9 cells. ( A ) Left: Volcano plot identified upregulated and downregulated genes, including mild downregulation of CXCL12 and upregulation of HOXB2, but no obvious differentiation factors or growth factors were identified. However, no obvious differentiation factors or cell growth factors were identified. Right: GO Gene ontology analysis showed upregulation of factors such as chemotaxis and extracellular matrix. KO cell. ( B ) Pathway enrichment analysis shows the pathways to which the differential genes are most related, with reference to the Gene Ontology database. ( C ) Heat map of the upregulated genes and downregulated genes between UE7T-9 cell control and UE7T-9 SRRM4 KO cell.

Article Snippet: A primary rabbit monoclonal antibody against anti-CXCL12 (clone D8G6H; Cell Signaling Technology, Danvers, MA, USA) was used in this study.

Techniques: Sequencing, Chemotaxis Assay, Control